Pristimantis yonke. (Credit: Germán Chávez)
In a nutshell
- Scientists discovered three new species of Pristimantis frogs in Peru’s Cordillera de Huancabamba, a remote and rugged mountain range rarely explored since the early 1990s.
- Each frog species has unique adaptations to distinct microhabitats, ranging from bromeliads in the canopy to rocky streams, highlighting the incredible biodiversity packed into small, high-altitude ecosystems.
- Despite the exciting discovery, habitat loss from agriculture, cattle ranching, and wildfires threatens the region; all three species are currently classified as Data Deficient because too little is known about their populations and distribution.
LIMA, Peru — Deep in the misty highlands of northwestern Peru, where ancient mountains pierce the clouds and few humans dare to tread, scientists have uncovered three entirely new species of frogs. These amphibians have been quietly living their lives in one of Earth’s most remote corners.
The newly discovered amphibians call the Cordillera de Huancabamba home, a rugged mountain range so treacherous that even seasoned researchers describe it as having “steep, exposed” ridges with “loose and muddy” soil where “the weather can be very changeable.” Apparently, it’s also where evolution has been busy cooking up new life forms, according to new research published in Evolutionary Systematics.
These three species were discovered in a region that has barely been explored by scientists since the early 1990s. Despite habitat loss in the area, the international research teams suspect there could be even more undiscovered species lurking in these remote peaks.
A High-Altitude Game of Hide-and-Seek
Finding new species in 2025 might seem unlikely, but scientists are still uncovering new life forms regularly, particularly in remote mountainous regions. The Cordillera de Huancabamba sits at elevations around 2,600 to 2,900 meters above sea level, roughly 8,500 to 9,500 feet up, where the air is thin and conditions can be harsh.
Each frog has adapted to its own specific environment. Pristimantis yonke, the smallest of the three new species, has made itself at home inside bromeliads—spiky, cup-shaped plants that collect water high up in the forest canopy. All specimens of this species were found 1-3 meters (3.3 to 9.8 feet) off the ground, tucked away in these natural water tanks.
Meanwhile, Pristimantis chinguelas prefers life on the rocky, exposed cliffsides, calling from leaves up to 1.8 meters high. Pristimantis nunezcortezi was discovered near rocky streams in secondary forest areas.
Lead researcher Germán Chávez and his team conducted multiple expeditions over several years, often working through both rainy and dry seasons. They walked 5-6 hours per night with headlamps to search different habitats.
DNA Analysis Confirms New Species
The research team used modern genetic analysis to confirm that these were indeed new species, not just variations of existing frogs. If two species differ by more than 3% in their DNA, they’re considered separate species, and some of these frogs had 4-6% differences from their nearest relatives.
The researchers found that many previously discovered species in this region haven’t been seen since they were first described or have only been spotted again after disappearing for many years. The research team estimates that nearly 50% of the amphibian species in this region are endemic, meaning they exist nowhere else on Earth.
The Meaning Behind Their Names
The researchers chose names that honor both the landscape and local culture. Pristimantis chinguelas gets its name from Cerro Chinguelas, the mountain where it was found, and where researchers note “a handful of explorers in the late ’70s started the scientific research in the Cordillera de Huancabamba.”
Pristimantis yonke is named after “yonke” or “yonque,” which the researchers describe as “a traditional drink made from sugar cane distillation and only drunk by local people from northwestern Andes to keep themselves warm during their journeys through the cold highlands, which may include night camps to get to other villages.”
The third species, Pristimantis nunezcortezi, honors Elio Nuñez-Cortez, a Peruvian ornithologist who the researchers say contributed to conservation efforts in the region.
Germán Chávez)
From 2001 to 2023, wildfires, farming, and cattle grazing spread further into the region, destroying almost 12,400 acres of natural habitat. Large wildfires hit the area again in November 2024, though researchers don’t yet know if these affected the newly discovered species’ habitats.
All three new species were classified as “Data Deficient” under conservation guidelines, meaning there simply isn’t enough information yet to determine how threatened they might be. The researchers estimate that Pristimantis chinguelas occupies an area smaller than 4 square miles, while only two Pristimantis nunezcortezi have been found, both at the same location.
The world’s species are fleeting in the face of climate change, but the discovery of new life forms is not yet lost. In remote regions like the Cordillera de Huancabamba, many more species could one day be discovered by scientists brave enough to go searching for them.
Paper Summary
Methodology
Researchers conducted field surveys across four sites in the Cordillera de Huancabamba in northwestern Peru between 2021 and 2024, during both rainy and dry seasons. They performed nocturnal Visual Encounter Surveys, typically walking 5-6 hours per night with four researchers systematically searching different habitats. They collected specimens, preserved them following standard protocols, and deposited them in the CORBIDI collection. The team used physical analysis and genetic analysis using 16S rRNA gene sequencing. They also recorded calls for one species using digital recording equipment.
Results
The research team discovered three new species of Pristimantis frogs. Pristimantis chinguelas is a medium-sized species found on rocky cliffsides. Pristimantis nunezcortezi is also medium-sized and found near rocky streams. Pristimantis yonke is the smallest and lives exclusively inside bromeliads 1-3 meters above ground. Genetic analysis confirmed all three as distinct species with DNA differences of 3-6% from their closest relatives. The species inhabit different environments at elevations between 2,593-2,949 meters above sea level.
Limitations
The study was limited by small sample sizes and restricted geographic sampling. All three species are known from very few specimens and limited locations, making it difficult to assess their true distribution ranges and population sizes. The genetic analysis used only a single gene, which provides less comprehensive evolutionary information than multi-gene approaches. Large portions of the region remain unexplored.
Funding and Disclosures
The fieldwork was supported by the Chicago Board of Trade (CBOT) Fund for Endangered Species and Nature and Culture International (NCI). Alessandro Catenazzi received support from the E.O. Wilson Biodiversity Foundation. Germán Chávez received support from Carlos Garnica from CANDES. Collecting permits were issued by Peru’s national forest and wildlife service.
Publication Information
This research, “Over the top: Three new species of terrestrial breeding frogs (Anura, Terrarana, Pristimantis) from the highlands of the Cordillera de Huancabamba, northwestern Peru,” was published in Evolutionary Systematics (Volume 9, pages 145-166). The paper was received on February 2, 2025, accepted on May 19, 2025, and published on June 20, 2025. The corresponding author is Germán Chávez from the Instituto Peruano de Herpetología in Lima, Peru.







