The sample locations where Curiosity snagged three samples of drilled rock at this site on its way out of the Glen Torridon region. Analyzing these samples revealed diverse organic molecules on Mars. (Credit: NASA/JPL-Caltech/MSSS)
In A Nutshell
- NASA’s Curiosity rover used a first-of-its-kind chemical experiment to unlock more than 20 organic molecules from a 3.5-billion-year-old Martian rock, the most diverse collection yet detected on Mars.
- Scientists used a lab chemical called TMAH to break apart larger carbon structures in the rock and free smaller molecules, including sulfur-, nitrogen-, and oxygen-bearing compounds.
- Organic material can survive more than three billion years of radiation and geological change on Mars, suggesting ancient rocks may still hold readable chemical records of the planet’s past.
- Researchers cannot yet determine whether the molecules came from meteorites, non-biological chemistry, or ancient life, but the results point the way for future missions to dig deeper.
On the 2,879th day of its mission, NASA’s Curiosity rover drilled into a 3.5-billion-year-old slab of clay-rich sandstone on Mars, doused the powder with a chemical reagent, heated it up, and pulled off a type of chemical experiment never before performed on another planetary body. What came out surprised even the scientists who built the experiment: more than 20 distinct organic molecules, one of the most diverse collections yet detected in Martian rock.
None of these molecules were sitting on the surface waiting to be found. They were trapped inside larger carbon structures embedded deep in the rock, like gold sealed inside a long-forgotten treasure box. The technique tore those structures apart, freeing smaller fragments the rover’s onboard lab could identify. Among them were compounds containing sulfur, nitrogen and oxygen, arranged in ring-shaped molecular architectures that scientists associate with long-lasting organic material.
Published in Nature Communications, the results do not prove life ever existed on Mars. But they confirm something nearly as important: that diverse organic material can survive more than three billion years of radiation exposure and geological change on a planet with no protective magnetic field. If future missions carry sharper tools, they may yet find signs of biology hiding in Mars’s ancient rocks, if any are there to find.
How Curiosity Performed a First-of-Its-Kind Organic Chemistry Test on Mars
At the center of the experiment was a chemical called TMAH, a reagent that Earth-based chemists routinely use to break apart large, stubborn organic structures that resist being vaporized on their own. It snips chemical bonds and attaches small molecular tags to the fragments, converting them into lighter molecules that instruments can identify.
Named after the famous 19th-century fossil hunter, the target rock was called Mary Anning 3, and it sat within Gale crater, a region that was once an ancient lakebed. Clay-rich minerals there are exactly the type scientists believe would preserve organic matter over billions of years.
Curiosity drilled the rock, delivered powdered stone into a sealed cup of TMAH, let the mixture soak for 19 minutes, then cranked the heat to about 550 degrees Celsius. Released gases were funneled into two analytical instruments: one that continuously sniffed the gases as they emerged, and another that separated and identified individual molecules.
More Than 20 Organic Molecules Found in Ancient Mars Rock
Seven molecules were confirmed by the rover’s onboard chemical analysis instruments and were absent in control runs performed without any rock sample or reagent. Identities were verified by matching chemical signatures against a standard reference library and comparing results to a near-identical instrument in a lab on Earth. Those seven ranged from single-ring carbon compounds to double-ring structures, present in tiny but clearly detectable quantities.
One molecule stood out among the confirmed seven. Benzothiophene, a compound built from a carbon ring fused with a sulfur-containing ring, had only been weakly hinted at in earlier Curiosity data. No known process inside the instrument is expected to produce it as a byproduct, and it is a well-known component of carbon-rich meteorites. Researchers believe it was freed from a larger sulfur-bearing structure native to the Martian rock.
Beyond those seven, the data showed 30 distinct signals tied to additional compounds. Many remain unidentified by name, but their chemical fingerprints revealed single- and double-ring carbon structures with methyl groups, nitrogen-containing side chains, oxygen-bearing branches and sulfur atoms.
What the Organic Molecules on Mars Might Mean
To make sense of where the Martian molecules might have come from, the team ran the same TMAH experiment on the Murchison meteorite, a carbon-rich space rock that fell in Australia in 1969. Sixteen of the 28 species confirmed or tentatively identified in the Mars experiment also appeared in the Murchison results.
Meteorite delivery is one possible source of the organics, but not the only one. Organics could also stem from non-biological chemical reactions between water and rock or, in a more tantalizing but unproven possibility, from ancient living organisms. Current data cannot distinguish among these sources.
One notable gap: fatty acids, which on Earth are strongly associated with the membranes of living cells, were not detected. But a timing problem in the instrument’s sampling sequence likely caused those signals to be lost before detection, rather than confirming they were absent from the rock. Researchers frame this as a lesson for optimizing the second TMAH cup still on Curiosity, as well as future experiments planned for the European Space Agency’s Rosalind Franklin rover and NASA’s Dragonfly mission to Saturn’s moon Titan.
Ancient Martian Sediments Still Hold Chemical Clues for Future Missions
What sets this result apart is not any single molecule but the sheer variety. Sulfur-bearing compounds, oxygen-carrying fragments, nitrogen-containing structures and multiple ring-shaped carbon molecules were all pulled from a single slab of 3.5-billion-year-old sandstone. That chemical diversity survived billions of years of radiation exposure and geological change, telling scientists that Mars’s ancient sedimentary rocks are not the radiation-sterilized wastelands some once feared. They are degraded but still readable records of a chemical past that future missions may finally be able to decode.
Paper Notes
Limitations
The study acknowledges several limitations. The internal reference compound was not detected due to losses caused by the gas venting sequence during the experiment, which lab testing confirmed was an artifact of the experimental design rather than a chemical failure. A second reference compound also could not be detected because it fell outside the heating windows of both detection channels. No fatty acid products were found, which the team attributes to these operational constraints rather than confirmed absence in the sample. Sixteen of the 30 signals in one detection channel remain unidentified by confirmed molecular name; their identities are inferred only from chemical fragment patterns and comparisons with reference databases. The distribution of organic matter within the sample cannot be determined by the rover’s instruments, and therefore the ultimate origin of the organics, whether delivered by meteorites, produced by water-rock chemistry, or generated biologically, remains unknown. Sample mass was estimated at approximately 163 ± 62 milligrams based on mineral and gas-release calculations, introducing uncertainty into abundance estimates. Saturation effects in one detection channel complicated separation of some compounds at higher temperatures.
Funding and Disclosures
Support for this work came from the NASA Mars Science Laboratory Participating Scientist Program (Grant #80NSSC22K0651), the CRESST II Cooperative Agreement (NASA Award Number 80GSFC21M0002), NASA-GSFC grant NNX17AJ68G, NASA’s Planetary Science Division Internal Scientist Funding Program through the Fundamental Laboratory Research work package at NASA Goddard Space Flight Center, the NASA Postdoctoral Program, the Carnegie Endowment, the University of Florida University Scholars Online Award, and CNES. Part of the research was carried out at the Jet Propulsion Laboratory under contract 80NM0018D0004 with NASA. Authors declare no competing interests.
Publication Details
Title: “Diverse organic molecules on Mars revealed by the first SAM TMAH experiment” | Journal: Nature Communications | DOI: https://doi.org/10.1038/s41467-026-70656-0 | Lead Author: Amy J. Williams, Department of Geological Sciences, University of Florida, Gainesville, FL. Corresponding author: [email protected] | Co-Authors: Jennifer L. Eigenbrode, Maëva Millan, Ross H. Williams, Ophélie M. Mcintosh, Samuel Teinturier, Janelle Roach, Charles Malespin, Amy C. McAdam, Paul Mahaffy, Alexander B. Bryk, Arnaud Buch, David Boulesteix, Luoth Chou, Jason P. Dworkin, Valerie Fox, Heather B. Franz, Caroline Freissinet, Daniel P. Glavin, Christopher H. House, Sarah Stewart Johnson, James M. T. Lewis, Angel Mojarro, Rafael Navarro-Gonzalez, Chad Pozarycki, Andrew Steele, Roger E. Summons, Cyril Szopa, Michael T. Thorpe, and Ashwin R. Vasavada. | Affiliated Institutions Include: NASA Goddard Space Flight Center; LATMOS/IPSL, Université Paris-Saclay; Georgetown University; University of Notre Dame; CentraleSupélec, University Paris-Saclay; University of California, Berkeley; University of Minnesota; The Pennsylvania State University; Howard University; Massachusetts Institute of Technology; Universidad Nacional Autónoma de México; Georgia Institute of Technology; Carnegie Institution for Science; University of Maryland; and Jet Propulsion Laboratory, California Institute of Technology. | Received: June 11, 2025. Accepted: March 2, 2026.







